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It was reported that an annual, transformable plant species, lettuce (), could synthesize and store NR in rubber particles in the same manner as that of the rubber tree (15).
In this work, we established lettuce as a model plant for the study of NR biosynthesis. Mariska and Ninja were obtained from the University of California Davis (Dr. Lettuce plants were grown in a growth chamber at 20 °C and a 16-h photoperiod for 30 days.
Considering the mechanistic commonality, it can be postulated that rubber synthases evolved from bacterial and eukaryotic -polyisoprenes longer than undecaprenol and dolichol has not been achieved using purified recombinant CPT enzymes.
It was recently identified that the human Nogo-B receptor (Ng BR) shares weak sequence homology to CPT (-glycosylation of proteins.
Quantitative real time PCR (q RT-PCR) was performed (Step One Real-Time PCR System; Applied Biosystems, Carlsbad, CA) using Power SYBER Green PCR Master Mix (Applied Biosystems), 5 μ primer, and c DNA template (equivalent to 5 ng of total RNA) in a reaction volume of 10 μl.
The q RT-PCR program was 1 cycle of 95 °C for 10 min and 40 cycles of 95 °C for 15 s and 58 °C for 1 min.
Undecaprenyl diphosphate (C55) and dolichyl diphosphate (C70–C110) function as essential polysaccharide carrier molecules during cell wall biosynthesis in bacteria and is not fully understood.
NR is distinguishable from undecaprenol and dolichol in that it has significantly longer polymer length and is not involved in essential primary metabolism.
Although TPT and CPT share a similar mechanism involving a carbocation intermediate, they share no sequence homology (5).Furthermore, yeast two-hybrid data showed that CPTL2 and CPT3 interact.Yeast microsomes containing CPTL2/CPT3 showed enhanced synthesis of short elasticity and abrasion resistance) required for many heavy duty products.NR belongs to the isoprenoid class of natural products, which use isopentenyl diphosphate (IPP) as a central building block.IPP and its isomer dimethylallyl diphosphate undergo condensations to generate C10 geranyl diphosphate, C15 farnesyl diphosphate (FPP), and C20 geranyl geranyl diphosphate (4).
RNA was isolated according to the manufacturer's protocol.